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Murali, T. S.
- Screening Marine-Derived Endophytic Fungi for Xylan-Degrading Enzymes
Abstract Views :283 |
PDF Views:90
Authors
N. Thirunavukkarasu
1,
Ben Jahnes
2,
Arthur Broadstock
3,
M. B. Govinda Rajulu
4,
T. S. Murali
5,
Venkat Gopalan
2,
T. S. Suryanarayanan
4
Affiliations
1 Department of Botany, Ramakrishna Mission Vivekananda College, Chennai 600 004, IN
2 Department of Microbiology, The Ohio State University, Columbus, OH 43210, US
3 Department of Chemistry and Biochemistry, The Ohio State University, Columbus, OH 43210, US
4 Vivekananda Institute of Tropical Mycology, Ramakrishna Mission Vidyapith, Chennai 600 004, IN
5 Department of Biotechnology, School of Life Sciences, Manipal University, Manipal 576 104, IN
1 Department of Botany, Ramakrishna Mission Vivekananda College, Chennai 600 004, IN
2 Department of Microbiology, The Ohio State University, Columbus, OH 43210, US
3 Department of Chemistry and Biochemistry, The Ohio State University, Columbus, OH 43210, US
4 Vivekananda Institute of Tropical Mycology, Ramakrishna Mission Vidyapith, Chennai 600 004, IN
5 Department of Biotechnology, School of Life Sciences, Manipal University, Manipal 576 104, IN
Source
Current Science, Vol 109, No 1 (2015), Pagination: 112-120Abstract
Marine-derived fungi surviving as symptomless endophytes in seaweeds and seagrasses were screened for production of xylan-degrading enzymes. Of the eight endophyte isolates obtained from five different seagrasses and another eight from six different marine algae, half of them exhibited xylanase activity in an agar plate assay. Further examination of these lead candidates using spectrophotometric assays revealed that Trichoderma harzianum, endophytic in the brown alga Sargassum wightii, had the maximum secreted xylanase and xylosidase activity. Moreover, this fungus could grow in NaCl-containing media (up to 1.2 M NaCl), and inclusion of 0.26 M NaCl in the media elicited a two- and three-fold increase in extracellular xylanase and xylosidase activity respectively. These findings highlight the potential of prospecting marine derived fungal endophytes to identify novel cell-wall degrading enzymes of value to the biofuel industry.Keywords
Biomass Deconstruction, Marine-Derived Fungi, Trichoderma harzianum, Xylan-Degrading Enzymes.Full Text
- The Host Range of Multi-Host Endophytic Fungi
Abstract Views :179 |
PDF Views:72
Authors
T. S. Suryanarayanan
1,
P. T. Devarajan
2,
K. P. Girivasan
3,
M. B. Govindarajulu
1,
V. Kumaresan
4,
T. S. Murali
5,
T. Rajamani
6,
N. Thirunavukkarasu
6,
G. Venkatesan
7
Affiliations
1 Vivekananda Institute of Tropical Mycology, RKM Vidyapith, Chennai 600 004, IN
2 Department of Plant Biology and Plant Biotechnology, Presidency College, Chennai 600 005, IN
3 Department of Botany, Government Arts College for Men, Nandanam, Chennai 600 035, IN
4 Department of Botany, Kanchi Mamunivar Centre for Post Graduate Studies, Puducherry 605 008, IN
5 Department of Biotechnology, School of Life Sciences, Manipal Academy of Higher Education, Manipal 576 104, IN
6 PG & Research Department of Botany, RKM Vivekananda College, Chennai 600 004, IN
7 Department of Botany, Mannai Rajagopalaswamy Government Arts College, Thanjavur 614 001, IN
1 Vivekananda Institute of Tropical Mycology, RKM Vidyapith, Chennai 600 004, IN
2 Department of Plant Biology and Plant Biotechnology, Presidency College, Chennai 600 005, IN
3 Department of Botany, Government Arts College for Men, Nandanam, Chennai 600 035, IN
4 Department of Botany, Kanchi Mamunivar Centre for Post Graduate Studies, Puducherry 605 008, IN
5 Department of Biotechnology, School of Life Sciences, Manipal Academy of Higher Education, Manipal 576 104, IN
6 PG & Research Department of Botany, RKM Vivekananda College, Chennai 600 004, IN
7 Department of Botany, Mannai Rajagopalaswamy Government Arts College, Thanjavur 614 001, IN
Source
Current Science, Vol 115, No 10 (2018), Pagination: 1963-1969Abstract
Mature leaves of 224 angiosperm plant species belonging to 60 families and growing in Andaman Islands, and the states of Arunachal Pradesh, Kerala and Tamil Nadu were sampled for the presence of endophytic fungi. Fungal genera such as Alternaria, Arthrinium, Aureobasidium, Chaetomium, Cladosporium, Glomerella/ Colletotrichum, Drechslera, Fusarium, Fusicoccum, Lasiodiplodia, Paecilomyces, Pestalotiopsis, Phoma, Diaporthe/Phomopsis, Guignardia/Phyllosticta, Sporormiella and Xylaria showed an isolation frequency of 5% or more. Species of Colletotrichum, Phyllosticta, Phomopsis and Xylaria occurred as endophytes in the leaves of many plant hosts including those that were taxonomically not closely related. The need to address the broad host range of some genera of fungal endophytes is discussed.Keywords
Diversity, Foliar Endophytes, Fungal Endophytes, Mutualism.Full Text
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- The fungal endobiome of seaweeds of the Andaman Islands, India
Abstract Views :167 |
PDF Views:69
Authors
Affiliations
1 Vivekananda Institute of Tropical Mycology, Ramakrishna Mission Vidyapith, Chennai 600 004, India, IN
2 Department of Biotechnology, Manipal School of Life Sciences, Manipal Academy of Higher Education, Manipal 576 104, India, IN
3 Jawaharlal Nehru Rajkeeya Mahavidyalaya College, Port Blair, Andaman and Nicobar Islands 744 101, India, IN
1 Vivekananda Institute of Tropical Mycology, Ramakrishna Mission Vidyapith, Chennai 600 004, India, IN
2 Department of Biotechnology, Manipal School of Life Sciences, Manipal Academy of Higher Education, Manipal 576 104, India, IN
3 Jawaharlal Nehru Rajkeeya Mahavidyalaya College, Port Blair, Andaman and Nicobar Islands 744 101, India, IN
Source
Current Science, Vol 123, No 12 (2022), Pagination: 1508-1514Abstract
Seventeen seaweed species (two green algae, nine brown algae and six red algae) of the Andaman Islands, India, were studied for their culturable fungal endophyte assemblage. A total of 796 endophytic isolates (67 species of fungi belonging to 22 genera and 10 sterile forms) were recovered from the 17 seaweeds. All the fungi were marine-derived forms and many belonged to Eurotiomycetes and Sordariomycetes of the Ascomycota group. More species of Aspergillus, Fusarium, Penicillium and Trichoderma were present as endophytes. While most endophytic species recovered were present in low frequency, some fungi like Aspergillus niger, Aspergillus sp. 1, Nodulisporium sp., Pestalotiopsis sp., Trichoderma yunnanense and Xylaria sp. 1 exhibited more than 40% frequency of colonization. Apart from yielding the maximum number of endophytic isolates, different Trichoderma species showed the highest colonization frequency in 11 of the 17 seaweeds. The results of this study indicate that fungi belonging to Eurotiomycetes which occur in low frequency as endophytes in terrestrial plants represent a significant percentage in the seaweeds and that the environment might have a more critical role than host specificity in determining the endophyte community of seaweed mycobiomeKeywords
Algal endophytes, eurotiomycetes, marine algae, Sordariomycetes, Trichoderma.Full Text
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